Absence of adenosine deaminase activity in a mammalian cell line transformed by Rous sarcoma virus

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Science  09 Jun 1978:
Vol. 200, Issue 4346, pp. 1163-1165
DOI: 10.1126/science.206965


No detectable adenosine deaminase activity was found in whole cells or 105,000g cytosol preparations of B-mix K-44/6 cells when either [3H]adenosine or [3H]arabinosyladenine was used as substrate. When grown in tissue culture medium supplemented with horse serum these cells provide a deaminase-free system not requiring the use of an adenosine deaminase inhibitor.