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Reversed-phase, high-pressure liquid chromatography of peptides and proteins with ion-pairing reagents

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Science  09 Jun 1978:
Vol. 200, Issue 4346, pp. 1168-1170
DOI: 10.1126/science.206966

Abstract

Reversed-phase, high-pressure liquid chromatography has been successfully applied to the analysis of peptides and proteins by the addition of hydrophilic (for example, phosphoric acid) or hydrophobic (for example, hexanesulfonic acid) ion-pairing reagents, or both, to the mobile phase. Examples described included proteins such as insulin, glucagon, and 1-24 ACTH pentaacetate (ACTH is adrenocorticotrophic hormone).