Cellular interactions uncouple beta-adrenergic receptors from adenylate cyclase

See allHide authors and affiliations

Science  17 Nov 1978:
Vol. 202, Issue 4369, pp. 765-768
DOI: 10.1126/science.213832


C6 glioma cells and B104 neuroblastoma cells both possess adenylate cyclase activity, but only C6 cells have beta-adrenergic receptors. However, when cocultured with B104 cells, C6 cells show a marked decrease in their ability to accumulate adenosine 3', 5'-monophosphate upon stimulation with beta receptor agonists. Since both beta receptors and cholera toxin-stimulated adenylate cyclase activities are present in C6/B104 cocultures, we conclude that the beta receptor/adenylate cyclase transduction mechanism in cocultured C6 cells is uncoupled.