Circadian clock in culture: N-acetyltransferase activity of chick pineal glands oscillates in vitro

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Science  16 Feb 1979:
Vol. 203, Issue 4381, pp. 656-658
DOI: 10.1126/science.569904


N-Acetyltransferase activity was measured in organ-cultured chick pineal glands. A circadian rhythm of enzyme activity persisted in cultured glands for up to 4 days. The phase of the rhythm in vitro closely approximates its phase in vivo. These observations demonstrate that the pineal gland of chicks contains (or is) a self-sustained circadian oscillator.