Molecular cloning of polyoma virus DNA in Escherichia coli: lambda phage vector system

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Science  02 Mar 1979:
Vol. 203, Issue 4383, pp. 887-892
DOI: 10.1126/science.217088


The biological activity of recombinant phage and recombinant phage DNA containing monomeric or dimeric polyoma DNA inserts was examined in mice and cultured mouse cells. Recombinant preparations containing a single copy of viral DNA were invariably noninfectious; molecules containing a dimeric polyoma DNA insert were at least seven orders of magnitude less infectious than polyoma virions after parenteral inoculation. No infection was detected with any recombinant preparation after oral administration.