Modified bases characterized in intact DNA by mass-analyzed ion kinetic energy spectrometry

Science  23 Mar 1979:
Vol. 203, Issue 4386, pp. 1249-1251
DOI: 10.1126/science.570725


Pyrolysis of DNA into a chemical ionization source yields protonated bases and other base-containing ions. Kinetic energy spectra allow the characterization of the bases 5-methylcytosine and 1-methyladenine from underivatized salmon sperm DNA. Isomeric bases are distinguishable with this technique.