Thiourea reverses cross-links and restores biological activity in DNA treated with dichlorodiaminoplatinum (II)

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Science  13 Apr 1979:
Vol. 204, Issue 4389, pp. 181-183
DOI: 10.1126/science.571145


Cis and trans dichlorodiaminoplatinum (II) compounds bind to DNA and form DNA cross-links, which are usually considered to be irreversible. Thiourea can reverse these cross-links without any apparent breakdown of the DNA. In addition, cis- and trans-Pt (II) treatment of lambda decreases its transfectivity. After suitable incubation with thiourea, full transfectivity of Pt(II)-treated lambda DNA can be restored.