Fluorescent retrograde double labeling: axonal branching in the ascending raphe and nigral projections

Science  25 May 1979:
Vol. 204, Issue 4395, pp. 873-875
DOI: 10.1126/science.441742


Red fluorescent Evans blue and blue fluorescent DAPI-primuline were injected into the anterior-medial and lateral-caudal forebrains, respectively, of the same rats. Separate clusters of cells labeled by retrograde transport were observed in the substantia nigra, while in the dorsal raphe many cells were double-labeled. Thus, single raphe cells send divergent axon collaterals to widespread forebrain areas.