Expression of the Escherichia coli cell division gene sep cloned in a lambda Charon phage

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Science  12 Oct 1979:
Vol. 206, Issue 4415, pp. 220-222
DOI: 10.1126/science.158216


The Escherichia coli cell division gene sep, which probably codes for one of the penicillin-binding proteins, has been cloned into lambda Charon 10 to form a viable sep+ transducing phage. After infection with this hybrid phage, penicillin-binding protein 3 was overproduced and incorporated into the E. coli inner membrane.