Circular forms of DNA synthesized by Rous sarcoma virus in vitro

See allHide authors and affiliations

Science  02 Nov 1979:
Vol. 206, Issue 4418, pp. 582-584
DOI: 10.1126/science.91199


Electron microscopic analysis of the DNA product synthesized by detergent-disrupted preparations of Rous sarcoma virus in vitro revealed the presence of several interesting molecular forms including covalently closed circular DNA. The identification of such circular DNA indicates that virions of retroviruses contain all the components necessary to facilitate the complete synthesis of mature forms of viral DNA and therefore provide a useful system to delineate the molecular mechanisms involved in their synthesis.