Pyruvate dehydrogenase activation in adipocyte mitochondria by an insulin-generated mediator from muscle

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Science  21 Dec 1979:
Vol. 206, Issue 4425, pp. 1407-1408
DOI: 10.1126/science.505013


Material in a chromatographic fraction from an extract of insulin-treated muscle stimulated pyruvate dehydrogenase activity in addipocyte mitochondria. This action was similar to insulin's activation of the enzyme in a plasma membrane-mitochondria mixture. Neither the chromatographic fraction nor insulin required adenosine triphosphate or magnesium ion (Mg2+), suggesting that both agents acted through a calcium-sensitive phosphatase. This fraction may contain a chemical mediator of insulin action.