Time: a new parameter for kinetic measurements in flow cytometry

See allHide authors and affiliations

Science  11 Jan 1980:
Vol. 207, Issue 4427, pp. 199-201
DOI: 10.1126/science.6153131


The measure of time was used as an additional parameter on an existing flow cytometer to study the kinetics of enzyme activities and cell-stain interactions. By correlating all fluorescent signals from single cells with time, the dynamics of a reaction can be followed for several minutes. This advanced application of flow cytometry is easily implemented and can be incorporated into any flow cytometer that has two-parameter analysis capability.