Cytosolic and microsomal epoxide hydrolases: differential properties in mammalian liver

Science  28 Mar 1980:
Vol. 207, Issue 4438, pp. 1479-1481
DOI: 10.1126/science.7361100


The epoxide hydrolase activities of the 100,000 g pellet (microsomal) and 100,00 g soluble (cystosolic) fractions of mouse, rat, and guinea pig liver were measured with three closely related compounds used as substrates. Differences between the species in the distribution of the cytosolic and microsomal hydrolases and in their substrate specificities and pH optima demonstrate why epoxide hydrolase activity in the cytosolic fraction was not detected earlier in spie of intensive work on the microsomal epoxide hydrolase.