Thyroid hormone receptor-containing fragment released from chromatin by deoxyribonuclease I and micrococcal nuclease

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Science  15 Aug 1980:
Vol. 209, Issue 4458, pp. 811-813
DOI: 10.1126/science.6250215


Limited deoxyribonuclease I and micrococcal nuclease digestion of hepatic nuclei from euthyroid rats injected with 125I-labeled triiodothyronine ([125I]T3) releases a discrete [125I]T3-labeled chromatin fragment (5.8S) which is larger than the T3 receptor (3.5S). These results suggest the T3 receptor is associated with a restricted fraction of hepatic chromatin that has a nuclease sensitivity characteristic of transcriptionally active chromatin.