Cultivation in vitro of the quartan malaria parasite Plasmodium inui

See allHide authors and affiliations

Science  12 Sep 1980:
Vol. 209, Issue 4462, pp. 1249-1251
DOI: 10.1126/science.6773146


The simian guartan malaria parasite Plasmodium inui (OS strain) was cultured in a continuous flow system with rhesus monkey erythrocytes and RPMI 1640nmedium supplemented with Hepes buffer and rhesus serum. Over a 10-week period, the growth of the parasite permitted a 61,000-fold cumulative dilution of the original inoculum. After 5 weeks in culture, the parasites were still infective to the monkey Saimiri sciureus and to Anopheles freeborni mosquitoes.