ArticlesStructures of the Genes That Rearrange in Development

Mapping of heavy chain genes for mouse immunoglobulins M and D

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Science  19 Sep 1980:
Vol. 209, Issue 4463, pp. 1348-1353
DOI: 10.1126/science.6774414


A single DNA fragment containing both mu and delta immunoglobulin heavy chain genes has been cloned from normal BALB/c mouse liver DNA with a new lambda phage vector Charon 28. The physical distance between the membrane terminal exon of mu and the first domain of delta is 2466 base pairs, with delta on the 3' side of mu. A single transcript could contain a variable region and both mu and delta constant regions. The dual expression of immunoglobulins M and D on spleen B cells may be due to alternate splicing of this transcript.