ArticlesExpression of Cloned Genes in New Environment

Expression of a bacterial gene in mammalian cells

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Science  19 Sep 1980:
Vol. 209, Issue 4463, pp. 1422-1427
DOI: 10.1126/science.6251549


Transfection of cultured monkey kidney cells with recombinant DNA constructed with a cloned Escherichia coli gene that codes for xanthine-guanine phosphoribosyltransferase and several different SV40 DNA-based vectors, results in the synthesis of readily measurable quantities of the bacterial enzyme. Moreover, the physiological defect in purine nucleotide synthesis characteristic of human Lesch-Nyhan cells can be overcome by the introduction of the bacterial gene into these cells.