ArticlesExpression of Cloned Genes in New Environment

A technique for expressing eukaryotic genes in bacteria

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Science  19 Sep 1980:
Vol. 209, Issue 4463, pp. 1428-1430
DOI: 10.1126/science.6158095


Methods are described that allow efficient expression in Escherichia coli of cloned eukaryotic genes. The methods require that the coding sequence of the gene in question be available in a form uninterrupted by intervening sequences (for example, as a complementary DNA clone). The gene products are synthesized unfused to other amino acid sequences. The genetic manipulations are simple, and require the plasmids described and commercially available enzymes.