Tumor promoter TPA mimics irradiation effects on the cell cycle of HeLa cells

Science  24 Oct 1980:
Vol. 210, Issue 4468, pp. 429-431
DOI: 10.1126/science.7433982


When asynchronous and synchronous HeLa cells were incubated with small doses (10(-7) M) of tumor promoter 12 O-tetradecanoylphorbol-13-acetate (TPA), a variety of transient alterations in the replication cycle were detected within 24 hours by the use of independent methods. Especially, a delayed passage through the S phase and influences on the G2 phase resemble x-ray irradiation effects on cell cultures. None of these alterations was observed with the hyperplasiogenic but nonpromoting 4-O-methyl-TPA.