Identification of tissue-specific nuclear antigens transferred to nitrocellulose from polyacrylamide gels

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Science  02 Jan 1981:
Vol. 211, Issue 4477, pp. 70-72
DOI: 10.1126/science.7003713


Nonhistone protein antigens resolved by electrophoresis in sodium dodecyl sulfate were identified immunochemically after being transferred to nitrocellulose. Use of antiserum to dehistonized chromatin from Novikoff hepatoma revealed numerous protein antigens specific to the chromatin of Novikoff hepatoma in comparison to that of normal rat liver.