Nematode development after removal of egg cytoplasm: absence of localized unbound determinants

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Science  23 Jan 1981:
Vol. 211, Issue 4480, pp. 402-405
DOI: 10.1126/science.7194506


Embryos of Caenorhabditis elegans develop into fertile adults after cell fragments, containing presumptive cytoplasm of somatic and germ line precursors, are extruded from uncleaved eggs or early blastomeres through laser-induced holes in the eggshells. This suggests that the determinate development of this worm is not dependent on the prelocalization of determinants in specific regions of the egg cytoplasm.