Two classes of single-stranded regions in DNA from sea urchin embryos

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Science  06 Feb 1981:
Vol. 211, Issue 4482, pp. 588-590
DOI: 10.1126/science.7455698


Native DNA from sea urchin embryos contains single-stranded regions (gaps) of up to 3000 nucleotides. The longer gaps (more than 1400 nucleotides) are nonrandomly distributed and are rich in histone gene sequences, other moderately repetitive sequences, and polypyrimidines. The shorter gaps are associated with DNA replication. A method for isolation of the two classes of single-stranded DNA pieces is reported.