Mutation caused by human phagocytes

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Science  01 May 1981:
Vol. 212, Issue 4494, pp. 546-547
DOI: 10.1126/science.6259738


Histidine-requiring mutants of Salmonella typhimurium TA100 were incubated with human peripheral blood leukocytes. More of these bacteria reverted to histidine independence than controls not incubated with cells. Phagocyte-rich suspensions were mutagenic, while heat-killed cells, lymphocytes, or mixed blood leukocytes of a patient with chronic granulomatous disease were not. Production of reactive oxygen metabolites could explain the capacity of phagocytes to induce mutation.