Hormonal Requirements for Growth of Arterial Smooth Muscle Cells in vitro: An Endocrine Approach to Atherosclerosis

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Science  15 May 1981:
Vol. 212, Issue 4496, pp. 818-820
DOI: 10.1126/science.7013068


In this study the hormonal requirements for the growth of arterial smooth muscle cells in vitro were determined. A serum-free, biochemically defined medium, supplemented with the relevant hormones, permitted proliferation and propagation of normal diploid mammalian arterial smooth muscle cells. Serum-free, hormone-supplemented cultures spontaneously formed atherosclerotic plaque-like nodules. Thus atherosclerosis may be mediated by a complex endocrine system.