Transmitter sensitivity of neurons assayed by autoradiography

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Science  22 May 1981:
Vol. 212, Issue 4497, pp. 929-930
DOI: 10.1126/science.6262911


Ionic conductance channels that are opened by activating nicotinic acetylcholine receptors at synapses of sympathetic neurons are permeable to small organic amines. Uptake of a tritium-labeled amine through these channels can be measured by autoradiography. This provides a simple and direct way to assess the sensitivity of individual neurons to acetylcholine without using microelectrodes.