Reports

Homogeneous fluorescent immunoassay

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Science  12 Jun 1981:
Vol. 212, Issue 4500, pp. 1266-1267
DOI: 10.1126/science.7015511

Abstract

A fluorescent immunoassay based on the correlation of fluctuations in particle number measures the amount of tagged species bound to micrometer-sized beads and is insensitive to background fluorescence. Without separation steps, a competitive assay can resolve I nanogram of gentamicin per milliliter from a total sample volume of only 10 microliters.

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