Myelination of central nervous system axons in tissue culture by transplanted oligodendrocytes

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Science  19 Jun 1981:
Vol. 212, Issue 4501, pp. 1407-1408
DOI: 10.1126/science.7233230


Unmyelinated mouse cerebellar cerebellar cultures in which oligodendrocyte differentiation had been suppressed by exposure to cytosine arabinoside developed axonal myelin after superimposition of kainic acid-treated cerebellar explants devoid of myelin-receptive axons. The latter explants contained differentiated oligodendrocytes. The operation of a diffusible myelin-stimulating factor was ruled out by the failure of myelination in cytosine arabinoside-exposed explants not in direct contact with oligodendrocyte-containing transplants.