Cloned poliovirus complementary DNA is infectious in mammalian cells

Science  20 Nov 1981:
Vol. 214, Issue 4523, pp. 916-919
DOI: 10.1126/science.6272391


A complete, cloned complementary DNA copy of the RNA genome of poliovirus was constructed in the Pst I site of the bacterial plasmid pBR322. Cultured mammalian cells transfected with this hybrid plasmid produced infectious poliovirus. Cells transfected with a plasmid which lacked the first 115 bases of the poliovirus genome did not produce virus.

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