Reports

Liposomes as gene carriers: efficient transformation of mouse L cells by thymidine kinase gene

Science  08 Jan 1982:
Vol. 215, Issue 4529, pp. 166-168
DOI: 10.1126/science.7053567

Abstract

Stable transformation of mouse L cells deficient in thymidine kinase was achieved by liposome-mediated transfer of a recombinant plasmid carrying the thymidine kinase gene. Ten percent of the recipient cells expressed thymidine kinase activity. The transformed phenotype (for example, 200 out of 10(6) cells) was stable under selective and nonselective conditions. The liposome technique is compared with other methods currently used for gene transfer.

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