Biosynthesis and processing of a human precursor complement protein, pro-C3, in a hepatoma-derived cell line

Science  22 Jan 1982:
Vol. 215, Issue 4531, pp. 399-400
DOI: 10.1126/science.7199205


A 180,000-dalton single-chain molecule (human pro-C3) is the precursor of the third component of human complement (C3), a disulfide-linked two-chain protein. The pro-C3 is converted by limited proteolysis to C3. The relationship between pro-C3 and C3 was established with the use of Hep G2, a cell line derived from a human hepatocellular carcinoma, which synthesizes at least 17 plasma proteins.