Inhibition of idiotype--anti-idiotype interaction for detection of a parasite antigen: a new immunoassay

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Science  26 Mar 1982:
Vol. 215, Issue 4540, pp. 1637-1639
DOI: 10.1126/science.6122269


Described in this report is an immunoradiometric assay of general applicability that is based on a new principle: the inhibition of the interaction between monoclonal antibodies by an antigen. The advantages of this assay are that it measures concentrations of single epitopes, purified antigen is not required, and the reagents can be obtained in unlimited amounts and are homogeneous. Its features are particularly attractive when the antigen has not been purified and is a minor component of a complex mixture of molecule.