Reports

Human aldehyde dehydrogenase: mechanism of inhibition of disulfiram

Science  07 May 1982:
Vol. 216, Issue 4546, pp. 637-639
DOI: 10.1126/science.7071604

Abstract

Disulfiram labeled with carbon-14 reacts specifically with human liver aldehyde dehydrogenase E1 with loss of catalytic activity and no incorporation of label. Carbon-14-labeled diethyldithiocarbamate is formed and the number of enzyme sulfhydryl groups decreases from 34 to 30 during this process.Activity is recovered by-mercaptoethanol but not by glutathione, the physiological reducing agent.