Secretion of newly taken-up ascorbic acid by adrenomedullary chromaffin cells

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Science  14 May 1982:
Vol. 216, Issue 4547, pp. 737-739
DOI: 10.1126/science.7079733


Primary cultures of bovine adrenomedullary cells accumulate carbon-14-labeled ascorbic acid through a saturable and energy-dependent process. The newly taken-up ascorbate is released concomitantly with catecholamines upon stimulation of chromaffin cell secretion. The release of ascorbate is Ca2+-dependent and mediated through activation of nicotinic receptors. These results indicate that exogenous ascorbate taken up into chromaffin cells is incorporated in situ into a secretable compartment, probably the catecholamine-containing chromaffin vesicles.