Deep-Sea Bacteria: Isolation in the Absence of Decompression

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Science  18 Jun 1982:
Vol. 216, Issue 4552, pp. 1315-1317
DOI: 10.1126/science.216.4552.1315


Sampling and pure culture isolation of deep-sea bacteria without loss of in situ pressure is required in order to determine the viability of decompressionsensitive strains. This was achieved by using a pressure-retaining sterilizable seawater sampling system in connection with a prepressurized hyperbaric isolation chamber. Rates of growth and substrate uptake of the majority of isolates showed highly barotolerant characteristics, while the remainder (4 out of 15) exhibited barophilic characteristics.