Active genes are sensitive to deoxyribonuclease I during metaphase

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Science  13 Aug 1982:
Vol. 217, Issue 4560, pp. 648-650
DOI: 10.1126/science.6283640


The active exogenous murine leukemia virus sequences of mouse cells growing in culture are preferentially digested by deoxyribonuclease I in metaphase chromosomes. As determined by nuclear nick translation, all of the gene sequences of these cells active during interphase are in a deoxyribonuclease I-sensitive conformation during metaphase. This method of nick translation can therefore be used to label chromosomes in situ in order to visualize the active regions of the genome.