Transformation of human leukocytes by cocultivation with an adult T cell leukemia virus producer cell line

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Science  20 Aug 1982:
Vol. 217, Issue 4561, pp. 737-739
DOI: 10.1126/science.6980467


The transmission of adult T cell leukemia virus, a human retrovirus, into fresh leukocytes from normal humans was examined. One of three virus-carrying cell lines, tested after being subjected to lethal x-irradiation, consistently transformed leukocytes from adult peripheral blood and umbilical cord blood. All the transformed cell lines expressed adult T cell leukemia virus-associated antigen, but transformed lines originating from adult and umbilical cord blood exhibited T cell and non-T, non-B cell surface natures, respectively. Efforts to transform human leukocytes with cell-free virus were unsuccessful.