Nucleotide sequence of the oncogene encoding the p21 transforming protein of Kirsten murine sarcoma virus

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Science  03 Sep 1982:
Vol. 217, Issue 4563, pp. 937-939
DOI: 10.1126/science.6287573


The transforming protein ofKirsten murine sarcoma virus (Ki-MuSV) is a virally encoded 21-kilodalton protein called p21 kis. The sequences encoding p21 kis were genetically localized to a 1.3-kilobase segment near the 5' end of the viral genome by assaying the capacity of a series of defined deletion mutants of molecularly cloned Ki-MuSV DNA to induce focal transformation of mouse cells. Nucleotide sequencing of a portion of this region has led to the identification of an open reading frame of 567 nucleotides coding for p21 kis protein.