Reports

Numbers of receptor sites from Scatchard graphs: facts and fantasies

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Science  24 Sep 1982:
Vol. 217, Issue 4566, pp. 1247-1249
DOI: 10.1126/science.6287580

Abstract

Data for ligand and receptor binding presented in the format of a Scatchard graph are compared with the same data shown as bound ligand plotted against the logarithm of free ligand. From this comparison it is apparent that extrapolations in the Scatchard graph to yield total number of receptor sites are generally not correct.