Sedimentation Field Flow Fractionation: Applications

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Science  08 Oct 1982:
Vol. 218, Issue 4568, pp. 121-127
DOI: 10.1126/science.218.4568.121


Sedimentation field flow fractionation is a powerful, new, high-resolution separation method for a wide variety of colloids, micelles, particulates, and soluble macromolecules of biological interest. Advances in instrumentation allow sedimentation field flow fractionation operation with rotor speeds up to 32,000 revolutions per minute (∼85,000 gravities), which permits separation of materials as small as 5 x 105 molecular weight, depending on sample density. Compared to conventional centrifugation techniques, the gentle, mass-separating sedimentation field flow fractionation method is capable of higher resolution in shorter times.