An estrogen-binding protein and endogenous ligand in Saccharomyces cerevisiae: possible hormone receptor system

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Science  15 Oct 1982:
Vol. 218, Issue 4569, pp. 297-298
DOI: 10.1126/science.6289434


A protein macromolecule in the cytosol of the unicellular eukaryotic yeast Saccharomyces cerevisiae selectively binds the vertebrate estrogen hormone 17 beta-estradiol with high affinity. Lipid extracts of the yeast cells or the conditioned growth medium yield a substance that can bind competitively to the tritiated estradiol-binding sites in the yeast and to mammalian estrogen receptors. These findings suggest that the binding protein may be a primitive hormone receptor and that the lipid-extractable substance represents the endogenous ligand.