Chromosome localization of highly repetitive human DNA's and amplified ribosomal DNA with restriction enzymes

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Science  28 Jan 1983:
Vol. 219, Issue 4583, pp. 395-397
DOI: 10.1126/science.6294832


Restriction endonucleases cut and partially removed DNA throughout fixed air-dried human metaphase chromosomes. Some enzymes produced a G-banding pattern; some revealed the presence of multiple chromosome-specific classes of highly repetitive DNA in C-band heterochromatin. Enzymes that produced the informative C-band patterns had recognition sequences that were four or five, but not six, base pairs long and did not contain a cytosine-guanine doublet. In both rat and human chromosomes, regions containing amplified ribosomal RNA genes were specifically removed by the restriction endonuclease Msp I.