Sodium currents in segments of human heart cells

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Science  15 Apr 1983:
Vol. 220, Issue 4594, pp. 320-321
DOI: 10.1126/science.6301004


Isolated human heart cells were partially drawn into the lumen of a plastic tube and cleaved at the partitioning tube wall by intraluminal suction pulses. The extraluminal segment (10 to 20 percent of the cell length) was suitable for intracellular perfusion and voltage clamp. The time and voltage dependence of the sodium current, and the responses to changes in driving force and channel blockers, illustrate the potential of these preparations as models for the study of membrane channels.