Bovine x mouse hybridomas that secrete bovine immunoglobulin G1

Science  29 Apr 1983:
Vol. 220, Issue 4596, pp. 522-524
DOI: 10.1126/science.6403985


The interspecific fusion of normal bovine lymphocytes with a nonsecreting mouse hybridoma produced stable cell lines secreting bovine immunoglobulins. One of these lines has continued to secrete immunoglobulin G1 (5 to 10 micrograms per milliliter) for over 16 months. The bovine x mouse hybrid cells can be expected to provide bovine monoclonal immunoglobulins for sequencing studies and for use as serological standards as well as to provide messenger RNA for cloning bovine immunoglobulin genes.