High-efficiency ligation and recombination of DNA fragments by vertebrate cells

Science  06 May 1983:
Vol. 220, Issue 4597, pp. 606-609
DOI: 10.1126/science.6301012


DNA-mediated gene transfer (transfection) is used to introduce specific genes into vertebrate cells. Events soon after transfection were quantitatively analyzed by determining the infectivity of the DNA from an avian retrovirus and of mixtures of subgenomic fragments of this DNA. The limiting step of transfection with two DNA molecules is the uptake by a single cell of both DNA's in a biologically active state. Transfected cells mediate ligation and recombination of physically unlinked DNA's at nearly 100 percent efficiency.