Dipetalonema viteae (Nematoda: Filarioidea): culture of third-stage larvae to young adults in vitro

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Science  08 Jul 1983:
Vol. 221, Issue 4606, pp. 161-163
DOI: 10.1126/science.6682998


Infective third-stage larvae of Dipetalonema viteae (Nematoda: Filarioidea) were cultured to young adults in a cell-free culture system. Third-stage larvae from the tick vector grew, developed, and molted twice in a medium containing NCTC 135 and Iscove's modified Dulbecco's medium supplemented with fetal bovine serum under a gas phase of 95 percent nitrogen and 5 percent carbon dioxide. The availability of such a culture system for filariids should facilitate studies of their immunology, biochemistry, and sensitivity to drugs.