Depletion of intracellular polyamines may alter DNA conformation in 9L rat brain tumor cells

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Science  22 Jul 1983:
Vol. 221, Issue 4608, pp. 368-370
DOI: 10.1126/science.6408733


Depletion of polyamines in 9L rat brain tumor cells by treatment with alpha-difluoromethylornithine dramatically altered DNA conformation as measured by viscoelastometry. The reduction of intracellular putrescine and spermidine concentrations to less than 5 percent of their concentrations in control cells decreased the sensitivity of 9L cell DNA to x-irradiation and increased the maximum viscoelastic retardation time of the DNA. Both of these phenomena were reversed by addition of exogenous putrescine.