Direct Embryogenesis from Mesophyll Cells of Orchardgrass

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Science  26 Aug 1983:
Vol. 221, Issue 4613, pp. 850-851
DOI: 10.1126/science.221.4613.850


Segments taken from the basal 15 to 20 millimeters of the two innermost leaves of an orchardgrass (Dactylis glomerata L.) genotype produced somatic (nonzygotic) embryos directly from mesophyll cells without an intervening callus when cultured on an agar medium with 30 micromolar 3,6-dichloro-o-anisic acid (dicamba). This demonstration of high-frequency embryogenesis from mesophyll cells in Gramineae is strong evidence for totipotency of the cells.