Immunocytochemically identified vasopressin neurons in culture show slow, calcium-dependent electrical responses

Science  09 Sep 1983:
Vol. 221, Issue 4615, pp. 1052-1054
DOI: 10.1126/science.6348947


From morphological characterization and intracellular recordings, monolayer cultures derived from fetal mouse hypothalami were found to include functionally differentiated peptide neurons, a number of which appear to contain vasopressin. These cells exhibited particular patterns of slow, calcium-dependent membrane depolarizations, resembling in their periodicity and duration the phasic activity of vasopressin neurons recorded extracellularly in vivo.