Segregation and mapping analysis of polymorphic HLA class I restriction fragments: detection of a novel fragment

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Science  07 Oct 1983:
Vol. 222, Issue 4619, pp. 72-74
DOI: 10.1126/science.6312559


An HLA-B7 complementary DNA clone was used as a hybridization probe to analyze the segregation pattern of polymorphic class I restriction fragments in several families whose HLA types had been determined by serological techniques. In one family in which a crossover in the HLA region had occurred, a specific genomic fragment was mapped with respect to the crossover. In another family, a novel genomic fragment present in one child and absent in all other family members was observed. With the exception of this novel fragment, all polymorphic class I fragments observed in this study segregated with a serologically defined parental haplotype, a result consistent with HLA linkage.