A Morphogenetically Competent Soybean Suspension Culture

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Science  11 Nov 1983:
Vol. 222, Issue 4624, pp. 632-634
DOI: 10.1126/science.222.4624.632


A morphogenetically competent suspension culture was derived from embryonic axes of Glycine max cv. Mitchell. The cultural history included visual selection for nonfriable, embryo-like structures, recurrent selection in a regime of 2,4-dichlorophenoxyacetic acid exposure and withdrawal, and the replacement of the nitrogen in a Murashige and Skoog salts-based medium with 20 millimolar ammonium citrate. The embryoids produced by this suspension are capable of completing plantlet development. The suspension can be maintained by serial subculture.